Before entry into the transfer room, it would be advisable for the researcher to wear a clean lab coat a sterile cap, use a face mask and rub his hands with ethanol. The method of cell culture is meritorious over other methods of culturing because it serves as the best way to analyse and understand the cell metabolism and effects of different chemical substances on the cellular responses.
Protoplast isolation from parent plant using any mechanical or enzymatic method. Phytoremediation still required more efforts to be applicable India. Thus, from the above account it is clear that unlike animals in which differentiation is irreversible usuallythe plants have such a quality that even highly mature and differentiated cells have an ability to revert back to meristematic state.
Somatic embryogenesis under in-vitro conditions was first of all observed by Steward et. A batch culture is a suspension culture in which cells grow in a finite volume of the culture medium and as a result, medium gradually depletes.
Moreover, agitation of such media reduces the diffusion gradient in the nutrient supply. Micropropagation is the practice of rapidly multiplying stock plant material to produce a large number of progeny plants, using modern plant tissue culture methods.
It is achieved either by another culture or by microspore pollen culture. Culture rooms or incubators where conditions of temperature, humidity and light etc.